The Mouse Brain PrimaCell™ 8: Normal Nerve Astrocytes is for rapid (7-10 days) isolation and growth of normal mouse nerve astrocytes from newborn or adult mouse brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Brain PrimaCell™ 8 System (Cat No. 2-82017):
Mouse Brain PrimaCell™ 8: Normal Nerve Astrocytes Protocols
Mouse Brain Tissue Preparation Buffer 8: Normal Nerve Astrocytes
Mouse Brain OptiTDS™ 8: Tissue Dissociation System
Mouse Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Brain PrimaCell™ 8: Normal Nerve Astrocytes Growth Medium
Mouse Brain PrimaCell™ 8: Normal Nerve Astrocytes Growth Supplements with Serum (for 500 ml medium)

References:

[1] Lukas TJ, Wang AL. Isolation and culture of astrocytes from the retina and optic nerve. Methods Mol Biol. 2012;814:105-15.
[2] Gerspacher C, Scheuber U, Schiera G, Proia P, Gygax D, Di Liegro I. The effect of cadmium on brain cells in culture. Int J Mol Med. 2009 Sep;24(3):311-8.
[3] Weinstein DE. Biological Isolation and purification of primary rodent astrocytes. Curr Protoc Neurosci. 2001 May;Chapter 3:Unit 3.5.