The Mouse Brain PrimaCell™ 9: Normal Nerve Microglia is for rapid (7-10 days) isolation and growth of normal mouse nerve microglia from newborn or adult mouse brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Brain PrimaCell™ 9 System (Cat No. 2-82018):
Mouse Brain PrimaCell™ 9: Normal Nerve Microglia Protocols
Mouse Brain Tissue Preparation Buffer 9: Normal Nerve Microglia
Mouse Brain OptiTDS™ 9: Tissue Dissociation System
Mouse Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Brain PrimaCell™ 9: Normal Nerve Microglia Growth Medium
Mouse Brain PrimaCell™9: Normal Nerve Microglia Growth Supplements with Serum (for 500 ml medium)

References:

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[3] Weinstein DE. Biological Isolation and purification of primary rodent astrocytes. Curr Protoc Neurosci. 2001 May;Chapter 3:Unit 3.5.