The Mouse Cervix PrimaCell™ 3: Normal Cervical Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal cervical smooth muscle cells from mouse cervix tissues. This system features an optimal condition of tissue dissociation system, Cervix OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Mouse Cervix PrimaCell™ 3 System (Cat No. 2-82025):
Mouse n Cervix PrimaCell™ 3 Normal Cervical Smooth Muscle Cells Protocols
Mouse Cervix Tissue Preparation Buffer 3: Normal Cervical Smooth Muscle Cells
Mouse Cervix OptiTDS™ 3: Tissue Dissociation System
Mouse Cervix FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Cervix PrimaCell™ 3: Normal Cervical Smooth Muscle Cells Growth Medium
Mouse Cervix PrimaCell™ 3: Normal Cervical Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Watari M, Watari H, DiSanto ME, Chacko S, Shi GP, Strauss JF 3rd. Pro-inflammatory cytokines induce expression of matrix-metabolizing enzymes in human cervical smooth muscle cells. Am J Pathol. 1999 Jun;154(6):1755-62.
[2] Slater DM, Astle S, Woodcock N, Chivers JE, de Wit NC, Thornton S, Vatish M, Newton R. Anti-inflammatory and relaxatory effects of prostaglandin E2 in myometrial smooth muscle. Mol Hum Reprod. 2006 Feb;12(2):89-97. Epub 2006 Feb 2.