The Mouse Embryo PrimaCell™ 2: Normal Fetal Dermal Fibroblasts is for rapid (7-10 days) isolation and growth of normal fetal dermal fibroblasts from mouse embryo tissues. This system features an optimal condition of tissue dissociation system, Embryo OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Embryo PrimaCell™ System 2 (Cat No. 2-82031):
Mouse Embryo PrimaCell™ 2: Normal Fetal Dermal Fibroblasts Protocols
Mouse Embryo Tissue Preparation Buffer 2: Normal Embryo Endothelial Cells
Mouse Embryo OptiTDS™ 2: Tissue Dissociation System
Mouse Embryo FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Embryo PrimaCell™ 2: Normal Fetal Dermal Fibroblasts Growth Medium
Mouse Embryo PrimaCell™ 2: Normal Fetal Dermal Fibroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Han Y, Chai J, Sun T, Li D, Tao R. Differentiation of human umbilical cord mesenchymal stem cells into dermal fibroblasts in vitro. Biochem Biophys Res Commun. 2011 Oct 7;413(4):561-5. Epub 2011 Sep 8.
[2] Hidalgo E, Bartolome R, Dominguez C. Cytotoxicity mechanisms of sodium hypochlorite in cultured human dermal fibroblasts and its bactericidal effectiveness. Chem Biol Interact. 2002 Mar 20;139(3):265-82.
[3] Root LL, Shipley GD. Human dermal fibroblasts express multiple bFGF and aFGF proteins. In Vitro Cell Dev Biol. 1991 Oct;27A(10):815-22.