The Mouse Embryo PrimaCell™ 3: Normal Fetal Epidermal Keratinocytes is for rapid (7-10 days) isolation and growth of normal fetal epidermal keratinocytes from mouse embryo tissues. This system features an optimal condition of tissue dissociation system, Embryo OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Embryo PrimaCell™ System 3 (Cat No. 2-82032):
Mouse Embryo PrimaCell™ 3: Normal Fetal Epidermal Keratinocytes Protocols
Mouse Embryo Tissue Preparation Buffer 2: Normal Embryo Endothelial Cells
Mouse Embryo OptiTDS™ 3: Tissue Dissociation System
Mouse Embryo FibrOut™ 3: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Embryo PrimaCell™ 3: Normal Fetal Epidermal Keratinocytes Growth Medium
Mouse Embryo PrimaCell™ 3: Normal Fetal Epidermal Keratinocytes Growth Supplements with Serum (for 500 ml medium)

References:

[1] Tran CT, Huynh DT, Gargiulo C, Nguyen PT, Tran TT, Huynh MT, Nguyen TT, Filgueira L, Strong DM. In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells: the Saigonese culture. Cell Tissue Bank. 2011 May;12(2):125-33. Epub 2010 Mar 27.
[2] Richards S, Leavesley D, Topping G, Upton Z. Development of defined media for the serum-free expansion of primary keratinocytes and human embryonic stem cells. Tissue Eng Part C Methods. 2008 Sep;14(3):221-32.
[3] Hitomi J, Maruyama K, Kikuchi Y, Nagasaki K, Yamaguchi K. Characterization of a new calcium-binding protein abundant in amniotic fluid, CAAF2, which is produced by fetal epidermal keratinocytes during embryogenesis. Biochem Biophys Res Commun. 1996 Nov 21;228(3):757-63.