The Mouse Endothelium PrimaCell™: Normal Vascular Endothelial Cells is for rapid (7-10 days) isolation and growth of normal vascular endothelial cells from mouse endothelial tissues. This system features an optimal condition of tissue dissociation system, Endothelium OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Endothelium PrimaCell™ System (Cat No. 2-82034):
Mouse Endothelium PrimaCell™: Normal Vascular Endothelial Cells Protocols
Mouse Endothelium Tissue Preparation Buffer: Normal Endothelium Endothelial Cells
Mouse Endothelium OptiTDS™: Tissue Dissociation System
Mouse Endothelium FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Endothelium PrimaCell™: Normal Vascular Endothelial Cells Growth Medium
Mouse Endothelium PrimaCell™: Normal Vascular Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

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[2] Olafsson IH, Vilhjalmsson DT, Thormodsson FR. Preparation of cultured human vascular cells. Methods Mol Biol. 2012;849:245-59.
[3] Nolte A, Hossfeld S, Schroeppel B, Mueller A, Stoll D, Walker T, Wendel HP, Krastev R. Impact of polyelectrolytes and their corresponding multilayers to human primary endothelial cells. J Biomater Appl. 2012 Mar 28.