The Mouse Heart PrimaCell™ 4: Normal Saphenous Vein Endothelial Cells is for rapid (7-10 days) isolation and growth of normal saphenous vein endothelial cells from mouse heart tissues. This system features an optimal condition of tissue dissociation system, Heart OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Heart PrimaCell™ 4 System (Cat No. 2-82049):
Mouse Heart PrimaCell™ 4: Normal Saphenous Vein Endothelial Cells Protocols
Mouse Heart Tissue Preparation Buffer 4: Normal Saphenous Vein Endothelial Cells
Mouse Heart OptiTDS™ 4: Tissue Dissociation System
Mouse Heart FibrOut™ 4: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Heart PrimaCell™ 4: Normal Saphenous Vein Endothelial Cells Growth Medium
Mouse Heart PrimaCell™ 4: Normal Saphenous Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Asbeutah AM, Asfar SK, Safar H, Oriowo MA, Elhagrassi I, Abu-Assi MA, Cameron JD, McGrath BP. In vivo and in vitro assessment of human saphenous vein wall changes. Open Cardiovasc Med J. 2007;1:15-21. Epub 2007 Aug 17.
[2] Ellis SG, Chen MS, Jia G, Luke M, Cassano J, Lytle B. Relation of polymorphisms in five genes to long-term aortocoronary saphenous vein graft patency. Am J Cardiol. 2007 Apr 15;99(8):1087-9. Epub 2007 Feb 27.