The Mouse Heart PrimaCell™ 5: Normal Ventricular Myocytes is for rapid (7-10 days) isolation and growth of normal ventricular myocytes from mouse heart tissues. This system features an optimal condition of tissue dissociation system, Heart OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Heart PrimaCell™ 5 System (Cat No. 2-82050):
Mouse Heart PrimaCell™ 5: Normal Ventricular Myocytes Protocols
Mouse Heart Tissue Preparation Buffer 5: Normal Ventricular Myocytes
Mouse Heart OptiTDS™ 5: Tissue Dissociation System
Mouse Heart FibrOut™ 5: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Heart PrimaCell™ 5: Normal Ventricular Myocytes Growth Medium
Mouse Heart PrimaCell™ 5: Normal Ventricular Myocytes Growth Supplements with Serum (for 500 ml medium)

References:

[1] Jiang X, Xu C, Wang Y, Gao L, Yan C, Li D, Sun H. β2-adrenoceptor transfection enhances contractile reserve of isolated rat ventricular myocytes exposed to chronic isoprenaline stimulation by improving β1-adrenoceptor responsiveness. J Recept Signal Transduct Res. 2012 Feb;32(1):36-41.
[2] Zhou YF, Zheng XW, Zhang GH, Zong ZH, Qi GX. The effect of hypoxia-inducible factor 1-alpha on hypoxia-induced apoptosis in primary neonatal rat ventricular myocytes. Cardiovasc J Afr. 2010 Jan-Feb;21(1):37-41.
[3] Seya K, Kanemaru K, Sugimoto C, Suzuki M, Takeo T, Motomura S, Kitahara H, Niwa M, Oshima Y, Furukawa K. Opposite effects of two resveratrol (trans-3,5,4'-trihydroxystilbene) tetramers, vitisin A and hopeaphenol, on apoptosis of myocytes isolated from adult rat heart. J Pharmacol Exp Ther. 2009 Jan;328(1):90-8. Epub 2008 Oct 16.