The Mouse Intestine PrimaCell™ 1: Normal Intestinal Mucosa Epithelial Cells is for rapid (7-10 days) isolation and growth of normal intestinal mucosa epithelial cells from mouse intestine tissues. This system features an optimal condition of tissue dissociation system, Intestine OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Intestine PrimaCell™ 1 System (Cat No. 2-82053):
Mouse Intestine PrimaCell™ 1: Normal Intestinal Mucosa Epithelial Cells Protocols
Mouse Intestine Tissue Preparation Buffer 1: Normal Intestinal Mucosa Epithelial Cells
Mouse Intestine OptiTDS™ 1: Tissue Dissociation System
Mouse Intestine FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Intestine PrimaCell™ 1: Normal Intestinal Mucosa Epithelial Cells Growth Medium
Mouse Intestine PrimaCell™ 1: Normal Intestinal Mucosa Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Pan D, Das A, Liu D, Veazey RS, Pahar B. Isolation and characterization of intestinal epithelial cells from normal and SIV-infected rhesus macaques. PLoS One. 2012;7(1):e30247. Epub 2012 Jan 26.
[2] Lahar N, Lei NY, Wang J, Jabaji Z, Tung SC, Joshi V, Lewis M, Stelzner M, Martín MG, Dunn JC. Intestinal subepithelial myofibroblasts support in vitro and in vivo growth of human small intestinal epithelium. PLoS One. 2011;6(11):e26898. Epub 2011 Nov 17.
[3] Wilhelm A, Jahns F, Böcker S, Mothes H, Greulich KO, Glei M. Culturing explanted colon crypts highly improves viability of primary non-transformed human colon epithelial cells. Toxicol In Vitro. 2012 Feb;26(1):133-41. Epub 2011 Oct 21.