The Mouse Intestine PrimaCell™ 3: Normal Intestinal Vein Endothelial Cells is for rapid (7-10 days) isolation and growth of normal intestinal vein endothelial cells from mouse intestine tissues. This system features an optimal condition of tissue dissociation system, Intestine OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Intestine PrimaCell™ 3 System (Cat No. 2-82055):
Mouse Intestine PrimaCell™ 3: Normal Intestinal Vein Endothelial Cells Protocols
Mouse Intestine Tissue Preparation Buffer 3: Normal Intestinal Vein Endothelial Cells
Mouse Intestine OptiTDS™ 3: Tissue Dissociation System
Mouse Intestine FibrOut™ 3: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Intestine PrimaCell™ 3: Normal Intestinal Vein Endothelial Cells Growth Medium
Mouse Intestine PrimaCell™ 3: Normal Intestinal Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

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[2] Shkilnyy A, Proulx P, Sharp J, Lepage M, Vermette P. Diffusion of rhodamine B and bovine serum albumin in fibrin gels seeded with primary endothelial cells. Colloids Surf B Biointerfaces. 2012 May 1;93:202-7. Epub 2012 Jan 13.
[3] Mikkelsen L, Jensen KA, Koponen IK, Saber AT, Wallin H, Loft S, Vogel U, Møller P. Cytotoxicity, oxidative stress and expression of adhesion molecules in human umbilical vein endothelial cells exposed to dust from paints with or without nanoparticles. Nanotoxicology. 2012 Jan 20.