The Mouse Kidney PrimaCell™ 3: Normal Renal Podocytes is for rapid (7-10 days) isolation and growth of normal renal podocytes from mouse kidney tissues. This system features an optimal condition of tissue dissociation system, Kidney OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Mouse Kidney PrimaCell™ 3 System (Cat No. 2-82058):
Mouse Kidney PrimaCell™ 3: Normal Renal Podocytes Protocols
Mouse Kidney Tissue Preparation Buffer 3: Normal Renal Podocytes
Mouse Kidney OptiTDS™ 3: Tissue Dissociation System
Mouse Kidney FibrOut™ 3: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Kidney PrimaCell™ 3: Normal Renal Podocytes Growth Medium
Mouse Kidney PrimaCell™ 3: Normal Renal Podocytes Growth Supplements with Serum (for 500 ml medium)
References:
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[2] Mikulak J, Teichberg S, Arora S, Kumar D, Yadav A, Salhan D, Pullagura S, Mathieson PW, Saleem MA, Singhal PC. DC-specific ICAM-3-grabbing nonintegrin mediates internalization of HIV-1 into human podocytes. Am J Physiol Renal Physiol. 2010 Sep;299(3):F664-73. Epub 2010 Jul 14.
[3] Krtil J, Pláteník J, Kazderová M, Tesar V, Zima T. Culture methods of glomerular podocytes. Kidney Blood Press Res. 2007;30(3):162-74. Review.