The Mouse Lung PrimaCell™ 4: Normal Pulmonary Artery Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal pulmonary artery smooth muscle cells from mouse lung tissues. This system features an optimal condition of tissue dissociation system, Lung OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Lung PrimaCell™ 4 System (Cat No. 2-82068):
Mouse Lung PrimaCell™ 4: Normal Pulmonary Artery Smooth Muscle Cells Protocols
Mouse Lung Tissue Preparation Buffer 4: Normal Pulmonary Artery Smooth Muscle Cells
Mouse Lung OptiTDS™ 4: Tissue Dissociation System
Mouse Lung FibrOut™ 4: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Lung PrimaCell™ 4: Normal Pulmonary Artery Smooth Muscle Cells Growth Medium
Mouse Lung PrimaCell™ 4: Normal Pulmonary Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Manetsch M, Che W, Seidel P, Chen Y, Ammit AJ. MKP-1: a negative feedback effector that represses MAPK-mediated pro-inflammatory signaling pathways and cytokine secretion in human airway smooth muscle cells. Cell Signal. 2012 Apr;24(4):907-13. Epub 2011 Dec 19.
[2] Billington CK, Hall IP. Real time analysis of β(2)-adrenoceptor-mediated signaling kinetics in human primary airway smooth muscle cells reveals both ligand and dose dependent differences. Respir Res. 2011 Jul 2;12:89.
[3] John TA. Effect of chronic high altitude hypoxia on foetal and maternal juxta-alveolar distal pulmonary smooth muscle cells actin and calponin organisation and growth profiles. West Afr J Med. 2010 Nov-Dec;29(6):388-92.