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Mouse Lung PrimaCell™6: Normal Pulmonary Vein Smooth Muscle Cells

The Mouse Lung PrimaCell™ 6: Normal Pulmonary Vein Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal pulmonary vein smooth muscle cells from mouse lung tissues. This system features an optimal condition of tissue dissociation system, Lung OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Lung PrimaCell™ 6 System (Cat No. 2-82070):
Mouse Lung PrimaCell™ 6: Normal Pulmonary Vein Smooth Muscle Cells Protocols
Mouse Lung Tissue Preparation Buffer 6: Normal Pulmonary Vein Smooth Muscle Cells
Mouse Lung OptiTDS™ 6: Tissue Dissociation System
Mouse Lung FibrOut™ 6: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Lung PrimaCell™ 6: Normal Pulmonary Vein Smooth Muscle Cells Growth Medium
Mouse Lung PrimaCell™ 6: Normal Pulmonary Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Manetsch M, Che W, Seidel P, Chen Y, Ammit AJ. MKP-1: a negative feedback effector that represses MAPK-mediated pro-inflammatory signaling pathways and cytokine secretion in human airway smooth muscle cells. Cell Signal. 2012 Apr;24(4):907-13. Epub 2011 Dec 19.
[2] Billington CK, Hall IP. Real time analysis of β(2)-adrenoceptor-mediated signaling kinetics in human primary airway smooth muscle cells reveals both ligand and dose dependent differences. Respir Res. 2011 Jul 2;12:89.
[3] John TA. Effect of chronic high altitude hypoxia on foetal and maternal juxta-alveolar distal pulmonary smooth muscle cells actin and calponin organisation and growth profiles. West Afr J Med. 2010 Nov-Dec;29(6):388-92.

Cat No. Description Unit Price Order
2-82070 Mouse Lung PrimaCell™6: Normal Pulmonary Vein Smooth Muscle Cells Kit $ 1039.50 
4-28184 Mouse Lung OptiTDS™6: Tissue Dissociation System Kit $ 255.15 
7-15164 Mouse Lung FibrOut™6: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 283.50 
9-32070 Mouse Lung PrimaCell™6: Normal Pulmonary Vein Smooth Muscle Cells Growth Medium 5 x 100 ml $ 122.85 
9-33070 Mouse Lung PrimaCell™6: Normal Pulmonary Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) set $ 283.50 
9-80184 Mouse Lung Tissue Preparation Buffer 6: Normal Pulmonary Vein Smooth Muscle Cells 1 x 100 ml $ 160.65 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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