Mouse Lung PrimaCell™™7: Alveolar Epithelial Cells II is for rapid (7-10 days) isolation and growth of normal alveolar epithelial cells type II from mouse lung tissues. This system features an optimal condition of tissue dissociation system Lung OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Lung PrimaCell™7 System (Cat No. 2-82101):
Mouse Lung PrimaCell™7 : Alveolar Epithelial Cells II Protocols
Mouse Lung Tissue Preparation Buffer 7: Alveolar Epithelial Cells II
Mouse Lung OptiTDS™7: Tissue Dissociation System
Mouse Lung FibrOut™7: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Lung PrimaCell™7: Alveolar Epithelial Cells II Growth Medium
Mouse Lung PrimaCell™7: Alveolar Epithelial Cells II Growth Supplements with Serum (for 500 ml medium)

References:

[1] Huang B, Zhuang Y, Li CQ, Liu LT, Zhou Y. Regeneration of the intervertebral disc with nucleus pulposus cell-seeded collagen II/hyaluronan/chondroitin-6-sulfate tri-copolymer constructs in a rabbit disc degeneration model. Spine (Phila Pa 1976). 2011 Dec 15;36(26):2252-9.
[2] Murai K, Sakai D, Nakamura Y, Nakai T, Igarashi T, Seo N, Murakami T, Kobayashi E, Mochida J. Primary immune system responders to nucleus pulposus cells: evidence for immune response in disc herniation. Eur Cell Mater. 2010 Jan 14;19:13-21.