The Mouse Lymph PrimaCell™ 2: Normal Lymphatic Blood Vessel Endothelial Cells is for rapid (7-10 days) isolation and growth of normal lymphatic blood vessel endothelial cells from mouse lymph tissues. This system features an optimal condition of tissue dissociation system, Lymph OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Mouse Lymph PrimaCell™ 2 System (Cat No. 2-82072):
Mouse Lymph PrimaCell™ 2: Normal Lymphatic Blood Vessel Endothelial Cells Protocols
Mouse Lymph Tissue Preparation Buffer 2: Normal Lymphatic Blood Vessel Endothelial Cells
Mouse Lymph OptiTDS™ 2: Tissue Dissociation System
Mouse Lymph FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Lymph PrimaCell™ 2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium
Mouse Lymph PrimaCell™ 2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Kinet V, Castermans K, Herkenne S, Maillard C, Blacher S, Lion M, Noël A, Martial JA, Struman I. The angiostatic protein 16K human prolactin significantly prevents tumor-induced lymphangiogenesis by affecting lymphatic endothelial cells. Endocrinology. 2011 Nov;152(11):4062-71. Epub 2011 Aug 23.
[2] Coso S, Zeng Y, Sooraj D, Williams ED. Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells. Exp Cell Res. 2011 Oct 15;317(17):2397-407. Epub 2011 Aug 2.
[3] Ji RC. Lymphatic endothelial cells, lymphedematous lymphangiogenesis, and molecular control of edema formation. Lymphat Res Biol. 2008;6(3-4):123-37. Review.