The Mouse Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells is for rapid (7-10 days) isolation and growth of normal skeletal muscle cells from mouse muscle tissues. This system features an optimal condition of tissue dissociation system, Muscle OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Mouse Muscle PrimaCell™ 1 System (Cat No. 2-82074):
Mouse Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells Protocols
Mouse Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells
Mouse Muscle OptiTDS™ 1: Tissue Dissociation System
Mouse Muscle FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells Growth Medium
Mouse Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)
References:
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[2] Tasnim F, Zink D. Cross talk between primary human Muscle cells and endothelial cells in cocultures. Am J Physiol Renal Physiol. 2012 Apr;302(8):F1055-62. Epub 2012 Feb 8.
[3] Domhan S, Ma L, Tai A, Anaya Z, Beheshti A, Zeier M, Hlatky L, Abdollahi A. Intercellular communication by exchange of cytoplasmic material via tunneling nano-tube like structures in primary human renal epithelial cells. PLoS One. 2011;6(6):e21283. Epub 2011 Jun 27.