The Mouse Ovary PrimaCell™ 1: Normal Ovarian Epithelial Cells is for rapid (7-10 days) isolation and growth of normal ovarian epithelial cells from mouse ovary tissues. This system features an optimal condition of tissue dissociation system, Ovary OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Ovary PrimaCell™ 1 System (Cat No. 2-82077):
Mouse Ovary PrimaCell™ 1: Normal Ovarian Epithelial Cells Protocols
Mouse Ovary Tissue Preparation Buffer 1: Normal Ovarian Epithelial Cells
Mouse Ovary OptiTDS™ 1: Tissue Dissociation System
Mouse Ovary FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Ovary PrimaCell™ 1: Normal Ovarian Epithelial Cells Growth Medium
Mouse Ovary PrimaCell™ 1: Normal Ovarian Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Galas JF. Primary culture of ovarian cells for research on cell interactions in the hormonal control of steroidogenesis. Methods Mol Biol. 2012;806:227-49.
[2] Behbakht K, Sill MW, Darcy KM, Rubin SC, Mannel RS, Waggoner S, Schilder RJ, Cai KQ, Godwin AK, Alpaugh RK. Phase II trial of the mTOR inhibitor, temsirolimus and evaluation of circulating tumor cells and tumor biomarkers in persistent and recurrent epithelial ovarian and primary peritoneal malignancies: a Gynecologic Oncology Group study. Gynecol Oncol. 2011 Oct;123(1):19-26. Epub 2011 Jul 12.
[3] Fotheringham S, Levanon K, Drapkin R. Ex vivo culture of primary human fallopian tube epithelial cells. J Vis Exp. 2011 May 9;(51). pii: 2728. doi: 10.3791/2728.