The Mouse Pancreas PrimaCell™1: Normal Pancreatic Islet Beta-cells is for rapid (7-10 days) isolation and growth of normal pancreatic islet beta-cells. cells from mouse pancreas tissues. This system features an optimal condition of tissue dissociation system, Pancreas OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Pancreas PrimaCell™1 System (Cat No. 2-82081):
Mouse Pancreas PrimaCell™1: Normal Pancreatic Islet Beta-cells. Cells Protocols
Mouse Pancreas Tissue Preparation Buffer 1: Normal Pancreatic Islet Beta-cells. Cells
Mouse Pancreas OptiTDS™1: Tissue Dissociation System
Mouse Pancreas FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Pancreas PrimaCell™1: Normal Pancreatic Islet Beta-cells. Cells Growth Medium
Mouse Pancreas PrimaCell™1: Normal Pancreatic Islet Beta-cells. Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Demozay D, Tsunekawa S, Briaud I, Shah R, Rhodes CJ. Specific glucose-induced control of insulin receptor substrate-2 expression is mediated via Ca2+-dependent calcineurin/NFAT signaling in primary pancreatic islet β-cells. Diabetes. 2011 Nov;60(11):2892-902. Epub 2011 Sep 22.
[2] Bellomo EA, Meur G, Rutter GA. Glucose regulates free cytosolic Zn²⁺ concentration, Slc39 (ZiP), and metallothionein gene expression in primary pancreatic islet β-cells. J Biol Chem. 2011 Jul 22;286(29):25778-89. Epub 2011 May 25.
[3] Wu HL, Wang Y, Zhang P, Li SF, Chen X, Chen YK, Li JG, Yang SM, Su YP, Wang JP, Chen B. Reversible immortalization of rat pancreatic β cells with a novel immortalizing and tamoxifen-mediated self-recombination tricistronic vector. J Biotechnol. 2011 Feb 10;151(3):231-41. Epub 2010 Dec 15.