The Mouse Rectal PrimaCell™: Normal Rectal Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal rectal smooth muscle cells from mouse rectal tissues. This system features an optimal condition of tissue dissociation system, Rectal OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Rectal PrimaCell™ System (Cat No. 2-82084):
Mouse Rectal PrimaCell™: Normal Rectal Smooth Muscle Cells Protocols
Mouse Rectal Tissue Preparation Buffer: Normal Rectal Smooth Muscle Cells
Mouse Rectal OptiTDS™: Tissue Dissociation System
Mouse Rectal FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Rectal PrimaCell™: Normal Rectal Smooth Muscle Cells Growth Medium
Mouse Rectal PrimaCell™: Normal Rectal Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Brent L, Stephens FD. Primary rectal ectasia. A quantitative study of smooth muscle cells in normal and hypertrophied human bowel. Prog Pediatr Surg. 1976;9:41-62.
[2] Sheng H, Wang J, Lim JY, Davitt C, Minnich SA, Hovde CJ. Internalization of Escherichia coli o157:h7 by bovine rectal epithelial cells. Front Microbiol. 2011;2:32. Epub 2011 Feb 22.