The Mouse Umbilical Cord PrimaCell™ : Normal Umbilical Vein Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal umbilical vein smooth muscle cells from mouse umbilical cord tissues. This system features an optimal condition of tissue dissociation system, Umbilical Cord OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Umbilical Cord PrimaCell™ System (Cat No. 2-82091):
Mouse Umbilical Cord PrimaCell™ : Normal Umbilical Vein Smooth Muscle Cells Protocols
Mouse Umbilical Cord Tissue Preparation Buffer : Normal Umbilical Vein Smooth Muscle Cells
Mouse Umbilical Cord OptiTDS™ : Tissue Dissociation System
Mouse Umbilical Cord FibrOut™ : Fibroblast Inhibitory System (for 500 ml medium)
Mouse Umbilical Cord PrimaCell™ : Normal Umbilical Vein Smooth Muscle Cells Growth Medium
Mouse Umbilical Cord PrimaCell™ : Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

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[2] Billington CK, Hall IP. Real time analysis of β(2)-adrenoceptor-mediated signaling kinetics in human primary airway smooth muscle cells reveals both ligand and dose dependent differences. Respir Res. 2011 Jul 2;12:89.
[3] John TA. Effect of chronic high altitude hypoxia on foetal and maternal juxta-alveolar distal pulmonary smooth muscle cells actin and calponin organisation and growth profiles. West Afr J Med. 2010 Nov-Dec;29(6):388-92.