The Mouse Urethral PrimaCell™: Normal Urethral Epithelial Cells is for rapid (7-10 days) isolation and growth of normal urethral epithelial cells from mouse urethral tissues. This system features an optimal condition of tissue dissociation system, Urethral OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Mouse Urethral PrimaCell™ System (Cat No. 2-82093):
Mouse Urethral PrimaCell™ : Normal Urethral Epithelial Cells Protocols
Mouse Urethral Tissue Preparation Buffer: Normal Urethral Epithelial Cells
Mouse Urethral OptiTDS™: Tissue Dissociation System
Mouse Urethral FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Urethral PrimaCell™: Normal Urethral Epithelial Cells Growth Medium
Mouse Urethral PrimaCell™: Normal Urethral Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Fu WJ, Wang ZX, Li G, Zhang BH, Zhang L, Hu K, Hong BF, Wang XX, Cui FZ, Zhang X. A surface-modified biodegradable urethral scaffold seeded with urethral epithelial cells. Chin Med J (Engl). 2011 Oct;124(19):3087-92.
[2] Harvey HA, Jennings MP, Campbell CA, Williams R, Apicella MA. Receptor-mediated endocytosis of Neisseria gonorrhoeae into primary human urethral epithelial cells: the role of the asialoglycoprotein receptor. Mol Microbiol. 2001 Nov;42(3):659-72.