The Rat Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells is for rapid (7-10 days) isolation and growth of normal rat bronchial epithelial (NHBE) cells from newborn or adult rat airway tissues. This system features an optimal condition of tissue dissociation system, Airway OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Airway PrimaCell™1 System (Cat No. 2-82501):
Rat Airway PrimaCell™1 Normal Bronchial and Tracheal Epithelial Cells Protocols
Rat Airway Tissue Preparation Buffer 1: Normal Bronchial and Tracheal Epithelial Cells
Rat Airway OptiTDS™1: Tissue Dissociation System
Rat Airway FibrOut™1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
Rat Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1]Lechnewr JF, LeVeck MA. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. Tissue Cult. Methods. 1985,9(2): 43-48.
[2] Rogers DF, Donnelly LE. Methods in Molecular Medicine, Human Airway Inflammation: Sampling Techniques and Analytical Protocols. Totowa: Humana Press Inc. 2000, 56: 127-136.
[3] Rowe RK, Brody SL, Pekosz A. Differentiated cultures of primary hamster tracheal airway epithelial cells reginak. In Vitro Cell Dev Biol Anim. 2004, 40(10): 303–311.