The Rat Airway PrimaCell™2: Normal Bronchial and Tracheal Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal rat bronchial smooth muscle cells from newborn or adult rat airway tissues. This system features an optimal condition of tissue dissociation system, Airway OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Airway PrimaCell™2 System (Cat No. 2-82502):
Rat Airway PrimaCell™2 Normal Bronchial and Tracheal Smooth Muscle Cells Protocols
Rat Airway Tissue Preparation Buffer 2: Normal Bronchial and Tracheal Smooth Muscle Cells
Rat Airway OptiTDS™2: Tissue Dissociation System
Rat Airway FibrOut™2: Fibroblast Inhibitory System (for 500 ml medium)
Rat Airway PrimaCell™2: Normal Bronchial and Tracheal Smooth Muscle Cells Growth Medium
Rat Airway PrimaCell™2: Normal Bronchial and Tracheal Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Malavia NK, Raub CB, Mahon SB, Brenner M, Panettieri RA Jr, George SC. Airway epithelium stimulates smooth muscle proliferation. Am J Respir Cell Mol Biol. 2009 Sep;41(3):297-304. Epub 2009 Jan 16.
[2] Martin JG, Ramos-Barbón D. Airway smooth muscle growth from the perspective of animal models. Respir Physiol Neurobiol. 2003 Sep 16;137(2-3):251-61. Review.
[3] Stengel J. Normal human bronchial tracheal cell system: an airway epithelial cell research tool. Am Biotechnol Lab. 1994 Mar;12(4):50-2.