The Rat Artery PrimaCell™: Normal Coronary Artery Endothelial Cells is for rapid (7-10 days) isolation and growth of normal rat coronary artery endothelial cells from newborn or adult rat artery tissues. This system features an optimal condition of tissue dissociation system, artery OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Artery PrimaCell™ System (Cat No. 2-82505):
Rat Artery PrimaCell™ Normal Coronary Artery Endothelial Cells Protocols
Rat Artery Tissue Preparation Buffer: Normal Coronary Artery Endothelial Cells
Rat Artery OptiTDS™: Tissue Dissociation System
Rat Artery FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Rat Artery PrimaCell™: Normal Coronary Artery Endothelial Cells Growth Medium
Rat Artery PrimaCell™: Normal Coronary Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Matsuda A, Morita H, Unno H, Saito H, Matsumoto K, Hirao Y, Munechika K, Abe J. Anti-inflammatory effects of high-dose IgG on TNF-α-activated human coronary artery endothelial cells Eur J Immunol. 2012 May 14. doi: 10.1002/eji.201242398.
[2] Grossini E, Caimmi P, Molinari C, Uberti F, Mary D, Vacca G. CCK receptors-related signaling involved in nitric oxide production caused by gastrin 17 in porcine coronary endothelial cells. Mol Cell Endocrinol. 2012 Mar 5;350(1):20-30. Epub 2011 Nov 25.
[3] Ding L, Ma W, Littmann T, Camp R, Shen J. The P2Y(2) nucleotide receptor mediates tissue factor expression in human coronary artery endothelial cells. J Biol Chem. 2011 Jul 29;286(30):27027-38. Epub 2011 Jun 7.