The Rat Brain PrimaCell™ 1: Normal Brain Fibroblasts is for rapid (7-10 days) isolation and growth of normal rat fibroblasts from newborn or adult rat brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Rat Brain PrimaCell™ 1 System (Cat No. 2-82511):
Rat Brain PrimaCell™ 1 Normal Fibroblasts Protocols
Rat Brain Tissue Preparation Buffer 1: Normal Fibroblasts
Rat Brain OptiTDS™ 1: Tissue Dissociation System
Rat Brain PrimaCell™ 1: Normal Fibroblasts Growth Medium
Rat Brain PrimaCell™ 1: Normal Fibroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Kadam SD, Gucek M, Cole RN, Watkins PA, Comi AM. Cell proliferation and oxidative stress pathways are modified in fibroblasts from Sturge-Weber syndrome patients Arch Dermatol Res. 2012 Apr;304(3):229-35. doi: 10.1007/s00403-012-1210-z. Epub 2012 Mar 10.
[2] Lee ST, Chu K, Jung KH, Song YM, Jeon D, Kim SU, Kim M, Lee SK, Roh JK Direct generation of neurosphere-like cells from human dermal fibroblasts. PLoS One. 2011;6(7):e21801. Epub 2011 Jul 13.