The Rat Brain PrimaCell™ 2: Normal Cerebellar Granule Cells is for rapid (7-10 days) isolation and growth of normal rat cerebellar granule cells from newborn or adult rat brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Brain PrimaCell™ 2 System (Cat No. 2-82512):
Rat Brain PrimaCell™ 2 Normal Cerebellar Granule Cells Protocols
Rat Brain Tissue Preparation Buffer 2: Normal Cerebellar Granule Cells
Rat Brain OptiTDS™ 2: Tissue Dissociation System
Rat Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Rat Brain PrimaCell™ 2: Normal Cerebellar Granule Cells Growth Medium
Rat Brain PrimaCell™ 2: Normal Cerebellar Granule Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Kadam Facci L, Skaper SD. Culture of rat cerebellar granule neurons and application to identify neuroprotective agents. Methods Mol Biol. 2012;846:23-37.
[2] Popp RL, Velasquez O, Bland J, Hughes P. Epub 2006 Mar 20. Characterization of protein kinase C isoforms in primary cultured cerebellar granule cells. Brain Res. 2006 Apr 14;1083(1):70-84. Epub 2006 Mar 20.