The Rat Brain PrimaCell™ 8: Normal Nerve Microglia is for rapid (7-10 days) isolation and growth of normal rat nerve microglia from newborn or adult rat brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Brain PrimaCell™ 8 System (Cat No. 2-82519):
Rat Brain PrimaCell™ 8: Normal Nerve Microglia Protocols
Rat Brain Tissue Preparation Buffer 8: Normal Nerve Microglia
Rat Brain OptiTDS™ 8: Tissue Dissociation System
Rat Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Rat Brain PrimaCell™ 8: Normal Nerve Microglia Growth Medium
Rat Brain PrimaCell™8: Normal Nerve Microglia Growth Supplements with Serum (for 500 ml medium)

References:

[1] Lukas TJ, Wang AL. Isolation and culture of astrocytes from the retina and optic nerve. Methods Mol Biol. 2012;814:105-15.
[2] Gerspacher C, Scheuber U, Schiera G, Proia P, Gygax D, Di Liegro I. The effect of cadmium on brain cells in culture. Int J Mol Med. 2009 Sep;24(3):311-8.
[3] Weinstein DE. Biological Isolation and purification of primary rodent astrocytes. Curr Protoc Neurosci. 2001 May;Chapter 3:Unit 3.5.