The Rat Cancer PrimaCell™ 3: Normal Cervical Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal cervical smooth muscle cells from rat cervical tissues. This system features an optimal condition of tissue dissociation system, Cervical OptiTDS™ that yields 6-8 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.
Rat Cancer PrimaCell™ 3 System (Cat No. 2-82526):
Rat Cancer PrimaCell™ 3:Normal Cervical Smooth Muscle Cells Protocols
Rat Cancer Tissue Preparation Buffer 3: Normal Cervical Smooth Muscle Cells
Rat Cancer OptiTDS™ 3: Tissue Dissociation System
Rat Cancer PrimaCell™ 3: Normal Cervical Smooth Muscle Cells Growth Medium
Rat Cancer PrimaCell™ 3: Normal Cervical Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Magaldi TG, Almstead LL, Bellone S, Prevatt EG, Santin AD, DiMaio D. Primary Human cervical carcinoma cells require Rat papillomavirus E6 and E7 expression for ongoing proliferation. Virology. 2012 Jan 5;422(1):114-24. Epub 2011 Nov 5.
[2] Feng D, Peng C, Li C, Zhou Y, Li M, Ling B, Wei H, Tian Z. Identification and characterization of cancer stem-like cells from primary carcinoma of the cervix uteri. Oncol Rep. 2009 Nov;22(5):1129-34.
[3] Beerens E, Van Renterghem L, Praet M, Sturtewagen Y, Weyers S, Temmerman M, Depypere H, Claeys P, Cuvelier CA. Rat papillomavirus DNA detection in women with primary abnormal cytology of the cervix: prevalence and distribution of HPV genotypes. Cytopathology. 2005 Aug;16(4):199-205.