The Rat Colon PrimaCell™1: Normal Colonic Epithelial Cells is for rapid (7-10 days) isolation and growth of normal colonic epithelial cells from rat colon tissues. This system features an optimal condition of tissue dissociation system, Colon OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Colon PrimaCell™ 1 System (Cat No. 2-82529):
Rat Colon PrimaCell™ 1: Normal Colonic Epithelial Cells Protocols
Rat Colon Tissue Preparation Buffer 1: Normal Colon Endothelial Cells
Rat Colon OptiTDS™ 1: Tissue Dissociation System
Rat Colon FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Colon PrimaCell™ 1: Normal Colonic Epithelial Cells Growth Medium
Rat Colon PrimaCell™ 1: Normal Colonic Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Wilhelm A, Jahns F, Böcker S, Mothes H, Greulich KO, Glei M. Culturing explanted colon crypts highly improves viability of primary non-transformed human colon epithelial cells. Toxicol In Vitro. 2012 Feb;26(1):133-41. Epub 2011 Oct 21.
[2] Petto C, Lesko S, Gäbel G, Böttner M, Wedel T, Kacza J, Pfannkuche H. Establishment and characterization of porcine colonic epithelial cells grown in primary culture. Cells Tissues Organs. 2011;194(6):457-68. Epub 2011 Mar 9.
[3] Hughes KR, Sablitzky F, Mahida YR. Expression profiling of Wnt family of genes in normal and inflammatory bowel disease primary human intestinal myofibroblasts and normal human colonic crypt epithelial cells. Inflamm Bowel Dis. 2011 Jan;17(1):213-20. doi: 10.1002/ibd.21353