The Rat Colon PrimaCell™ 2:Normal Colorectal Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of rat colorectal smooth muscle cells from rat colorectal tumor tissues. This system features an optimal condition of tissue dissociation system, Colon OptiTDS™ that yields 6-8 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.
Rat Cancer PrimaCell™ 2 System (Cat No. 2-82530):
Rat Cancer PrimaCell™ 2: Normal Colorectal Smooth Muscle Cells Protocols
Rat Cancer Tissue Preparation Buffer 2: Normal Colorectal Smooth Muscle Cells
Rat Cancer OptiTDS™ 2: Tissue Dissociation System
Rat Cancer PrimaCell™ 2:Normal Colorectal Smooth Muscle Cells Growth Medium
Rat Cancer PrimaCell™ 2:Normal Colorectal Smooth Muscle Cells Growth Supplements with Serum (for 600 ml medium)
References:
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[2] Desitter I, Guerrouahen BS, Benali-Furet N, Wechsler J, Jänne PA, Kuang Y, Yanagita M, Wang L, Berkowitz JA, Distel RJ, Cayre YE. A new device for rapid isolation by size and characterization of rare circulating tumor cells. Anticancer Res. 2011 Feb;31(2):427-41.
[3] Brouquet A, Taleb P, Lot AS, Beauchet A, Julie C, Prevost G, Nordlinger B, Penna C. A model of primary culture of colorectal cancer and liver metastasis to predict chemosensitivity. J Surg Res. 2011 Apr;166(2):247-54. Epub 2009 May 26.