The Rat Embryo PrimaCell™ 1: Normal Embryonic Chorion Cells is for rapid (7-10 days) isolation and growth of normal embryonic chorion cells from rat embryo tissues. This system features an optimal condition of tissue dissociation system, Embryo OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Embryo PrimaCell™ System 1 (Cat No. 2-82531):
Rat Embryo PrimaCell™ 1: Normal Embryonic Chorion Cells Protocols
Rat Embryo Tissue Preparation Buffer 1: Normal Embryo Endothelial Cells
Rat Embryo OptiTDS™ 1: Tissue Dissociation System
Rat Embryo FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Embryo PrimaCell™ 1: Normal Embryonic Chorion Cells Growth Medium
Rat Embryo PrimaCell™ 1: Normal Embryonic Chorion Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Yoshino Y, Yuan B, Kaise T, Takeichi M, Tanaka S, Hirano T, Kroetz DL, Toyoda H. Contribution of aquaporin 9 and multidrug resistance-associated protein 2 to differential sensitivity to arsenite between primary cultured chorion and amnion cells prepared from human fetal membranes. Toxicol Appl Pharmacol. 2011 Dec 1;257(2):198-208. Epub 2011 Sep 16.
[2] Yuan B, Ohyama K, Takeichi M, Toyoda H. Direct contribution of inducible nitric oxide synthase expression to apoptosis induction in primary smooth chorion trophoblast cells of human fetal membrane tissues. Int J Biochem Cell Biol. 2009 May;41(5):1062-9. Epub 2008 Oct 17.
[3] Uchide N, Suzuki A, Ohyama K, Bessho T, Toyoda H. Secretion of bioactive interleukin-6 and tumor necrosis factor-alpha proteins from primary cultured human fetal membrane chorion cells infected with influenza virus. Placenta. 2006 Jun-Jul;27(6-7):678-90. Epub 2005 Aug 24.