The Rat Embryo PrimaCell™ 4: Normal Trophoblast Cells is for rapid (7-10 days) isolation and growth of normal trophoblast cells from rat embryo tissues. This system features an optimal condition of tissue dissociation system, Embryo OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Embryo PrimaCell™ System 4 (Cat No. 2-82534):
Rat Embryo PrimaCell™ 4: Normal Trophoblast Cells Protocols
Rat Embryo Tissue Preparation Buffer 4: Normal Embryo Endothelial Cells
Rat Embryo OptiTDS™ 4: Tissue Dissociation System
Rat Embryo FibrOut™ 4: Fibroblast Inhibitory System (for 500 ml medium)
Rat Embryo PrimaCell™ 4: Normal Trophoblast Cells Growth Medium
Rat Embryo PrimaCell™ 4: Normal Trophoblast Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Donker RB, Mouillet JF, Chu T, Hubel CA, Stolz DB, Morelli AE, Sadovsky Y. The expression profile of C19MC microRNAs in primary human trophoblast cells and exosomes. Mol Hum Reprod. 2012 Mar 15.
[2] Oh SY, Chu T, Sadovsky Y. The timing and duration of hypoxia determine gene expression patterns in cultured human trophoblasts. Placenta. 2011 Dec;32(12):1004-9. Epub 2011 Oct 8.
[3] Lager S, Jansson N, Olsson AL, Wennergren M, Jansson T, Powell TL. Effect of IL-6 and TNF-α on fatty acid uptake in cultured human primary trophoblast cells. Placenta. 2011 Feb;32(2):121-7. Epub 2010 Dec 7.