The Rat Esophagus PrimaCell™ 1: Normal Esophageal Epithelial Cells is for rapid (7-10 days) isolation and growth of normal esophageal epithelial cells from rat esophagus tissues. This system features an optimal condition of tissue dissociation system, Esophagus OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Esophagus PrimaCell™ 1 System (Cat No. 2-82536):
Rat Esophagus PrimaCell™ 1: Normal Esophageal Epithelial Cells Protocols
Rat Esophagus Tissue Preparation Buffer 1: Normal Esophagus Epithelial Cells
Rat Esophagus OptiTDS™ 1: Tissue Dissociation System
Rat Esophagus FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Esophagus PrimaCell™ 1: Normal Esophageal Epithelial Cells Growth Medium
Rat Esophagus PrimaCell™ 1: Normal Esophageal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Isohata N, Aoyagi K, Mabuchi T, Daiko H, Fukaya M, Ohta H, Ogawa K, Yoshida T, Sasaki H. Hedgehog and epithelial-mesenchymal transition signaling in normal and malignant epithelial cells of the esophagus. Int J Cancer. 2009 Sep 1;125(5):1212-21.
[2] Kim SH, Nakagawa H, Navaraj A, Naomoto Y, Klein-Szanto AJ, Rustgi AK, El-Deiry WS. Tumorigenic conversion of primary human esophageal epithelial cells using oncogene combinations in the absence of exogenous Ras. Cancer Res. 2006 Nov 1;66(21):10415-24.
[3] Kim SH, Kim K, Kwagh JG, Dicker DT, Herlyn M, Rustgi AK, Chen Y, El-Deiry WS. Death induction by recombinant native TRAIL and its prevention by a caspase 9 inhibitor in primary human esophageal epithelial cells. J Biol Chem. 2004 Sep 17;279(38):40044-52. Epub 2004 Jun 28..