The Rat Eye PrimaCell™ 1: Normal Trabecular Meshwork Cells is for rapid (7-10 days) isolation and growth of normal trabecular meshwork cells from rat eye tissues. This system features an optimal condition of tissue dissociation system, Eye OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Eye PrimaCell™ 1 System (Cat No. 2-82538):
Rat Eye PrimaCell™ 1: Normal Trabecular Meshwork Cells Protocols
Rat Eye Tissue Preparation Buffer 2: Normal Trabecular Meshwork Cells
Rat Eye OptiTDS™ 1: Tissue Dissociation System
Rat Eye FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Eye PrimaCell™ 1: Normal Trabecular Meshwork Cells Growth Medium
Rat Eye PrimaCell™ 1: Normal Trabecular Meshwork Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Guo MS, Wu YY, Liang ZB. Hyaluronic acid increases MMP-2 and MMP-9 expressions in cultured trabecular meshwork cells from patients with primary open-angle glaucoma. Mol Vis. 2012;18:1175-81. Epub 2012 May 4.
[2] Paylakhi SH, Fan JB, Mehrabian M, Sadeghizadeh M, Yazdani S, Katanforoush A, Kanavi MR, Ronaghi M, Elahi E. Effect of PITX2 knockdown on transcriptome of primary human trabecular meshwork cell cultures. Mol Vis. 2011;17:1209-21. Epub 2011 May 5.
[3] Kahook MY, Ammar DA. In vitro effects of antivascular endothelial growth factors on cultured human trabecular meshwork cells. J Glaucoma. 2010 Sep;19(7):437-41.