The Rat Eye PrimaCell™ 2: Normal Corneal Epithelial Cells is for rapid (7-10 days) isolation and growth of normal corneal epithelial cells from rat eye tissues. This system features an optimal condition of tissue dissociation system, Eye OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Eye PrimaCell™ 2 System (Cat No. 2-82539):
Rat Eye PrimaCell™ 2: Normal Corneal Epithelial Cells Protocols
Rat Eye Tissue Preparation Buffer 2: Normal Corneal Epithelial Cells
Rat Eye OptiTDS™ 2: Tissue Dissociation System
Rat Eye FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Rat Eye PrimaCell™ 2: Normal Corneal Epithelial Cells Growth Medium
Rat Eye PrimaCell™ 2: Normal Corneal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Kim SW, Seo KY, Rhim T, Kim EK. Effect of retinoic acid on epithelial differentiation and mucin expression in primary human corneal limbal epithelial cells. Curr Eye Res. 2012 Jan;37(1):33-42. Epub 2011 Oct 26. Erratum in: Curr Eye Res. 2012 Jan;37(1):42.
[2] Oliveira LA, Kim C, Sousa LB, Schwab IR, Rosenblatt MI. Gene transfer to primary corneal epithelial cells with an integrating lentiviral vector. Arq Bras Oftalmol. 2010 Sep-Oct;73(5):447-53.
[3] Seaman CW, Whittingham A, Guest R, Warren N, Olson MJ, Guerriero FJ, Adriaens E, De Wever B. An evaluation of a cultured human corneal epithelial tissue model for the determination of the ocular irritation potential of pharmaceutical process materials. Toxicol In Vitro. 2010 Sep;24(6):1862-70. Epub 2010 Mar 17.