The Rat Eye PrimaCell™ 3: Normal Corneal Fibroblasts is for rapid (7-10 days) isolation and growth of normal corneal fibroblasts from rat eye tissues. This system features an optimal condition of tissue dissociation system, Eye OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Rat Eye PrimaCell™ 3 System (Cat No. 2-82540):
Rat Eye PrimaCell™ 3: Normal Corneal Fibroblasts Protocols
Rat Eye Tissue Preparation Buffer 3: Normal Corneal Fibroblasts
Rat Eye OptiTDS™ 3: Tissue Dissociation System
Rat Eye PrimaCell™ 3: Normal Corneal Fibroblasts Growth Medium
Rat Eye PrimaCell™ 3: Normal Corneal Fibroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Liu L, Walker EA, Kissane S, Khan I, Murray PI, Rauz S, Wallace GR. Gene expression and miR profiles of human corneal fibroblasts in response to dexamethasone. Invest Ophthalmol Vis Sci. 2011 Sep 21;52(10):7282-8. Print 2011 Sep.
[2] Wong Y, Sethu C, Louafi F, Hossain P. Lipopolysaccharide regulation of toll-like receptor-4 and matrix metalloprotease-9 in human primary corneal fibroblasts. Invest Ophthalmol Vis Sci. 2011 Apr 25;52(5):2796-803. Print 2011 Apr.
[3] Choi SI, Kim TI, Kim KS, Kim BY, Ahn SY, Cho HJ, Lee HK, Cho HS, Kim EK. Decreased catalase expression and increased susceptibility to oxidative stress in primary cultured corneal fibroblasts from patients with granular corneal dystrophy type II. Am J Pathol. 2009 Jul;175(1):248-61. Epub 2009 Jun 4.