The Rat Eye PrimaCell™ 5: Normal Retinal Pigment Epithelial Cells is for rapid (7-10 days) isolation and growth of normal retinal pigment epithelial Cells from rat eye tissues. This system features an optimal condition of tissue dissociation system, Eye OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Eye PrimaCell™ 5 System (Cat No. 2-82542):
Rat Eye PrimaCell™ 5: Normal Retinal Pigment Epithelial Cells Protocols
Rat Eye Tissue Preparation Buffer 5: Normal Retinal Pigment Epithelial Cells
Rat Eye OptiTDS™ 5: Tissue Dissociation System
Rat Eye FibrOut™ 5: Fibroblast Inhibitory System (for 500 ml medium)
Rat Eye PrimaCell™ 5: Normal Retinal Pigment Epithelial Cells Growth Medium
Rat Eye PrimaCell™ 5: Normal Retinal Pigment Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Bandyopadhyay M, Rohrer B. Matrix metalloproteinase activity creates pro-angiogenic environment in primary human retinal pigment epithelial cells exposed to complement. Invest Ophthalmol Vis Sci. 2012 Apr 18;53(4):1953-61. Print 2012 Apr.
[2] Chung EJ, Chun JN, Jung SA, Cho JW, Lee JH. TGF-β-stimulated aberrant expression of class III β-tubulin via the ERK signaling pathway in cultured retinal pigment epithelial cells. Biochem Biophys Res Commun. 2011 Nov 18;415(2):367-72. Epub 2011 Oct 20.
[3] Yu AL, Lorenz RL, Haritoglou C, Kampik A, Welge-Lussen U. Biological effects of native and oxidized low-density lipoproteins in cultured human retinal pigment epithelial cells. Exp Eye Res. 2009 Mar;88(3):495-503. Epub 2008 Nov 27.