The Rat Fat PrimaCell™ 1: Normal Adipocytes is for rapid (7-10 days) isolation and growth of normal adipocytes from rat fat tissues. This system features an optimal condition of tissue dissociation system, Fat OptiTDS™ that yields 1-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Rat Fat PrimaCell™ 1 System (Cat No. 2-82543):
Rat Fat PrimaCell™ 1: Normal Adipocytes Protocols
Rat Fat Tissue Preparation Buffer 1: Normal Adipocytes
Rat Fat OptiTDS™ 1: Tissue Dissociation System
Rat Fat FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Fat PrimaCell™ 1: Normal Adipocytes Growth Medium
Rat Fat PrimaCell™ 1: Normal Adipocytes Growth Supplements with Serum (for 500 ml medium)
References:
[1] Sargis RM, Neel BA, Brock CO, Lin Y, Hickey AT, Carlton DA, Brady MJ. The novel endocrine disruptor tolylfluanid impairs insulin signaling in primary rodent and human adipocytes through a reduction in insulin receptor substrate-1 levels. Biochim Biophys Acta. 2012 Jun;1822(6):952-60. Epub 2012 Feb 23.
[2] Ding Y, Wu Y, Zeng R, Liao K. Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse. Acta Biochim Biophys Sin (Shanghai). 2012 May;44(5):394-406. doi: 10.1093/abbs/gms008. Epub 2012 Feb 16.
[3] Ho JN, Jang JY, Yoon HG, Kim Y, Kim S, Jun W, Lee J. Anti-obesity effect of a standardised ethanol extract from Curcuma longa L. fermented with Aspergillus oryzae in ob/ob mice and primary mouse adipocytes. J Sci Food Agric. 2012 Jul;92(9):1833-40. doi: 10.1002/jsfa.5592. Epub 2012 Jan 25.