The Rat Fat PrimaCell™ 2: Normal Subcutaneous Fat Cells is for rapid (7-10 days) isolation and growth of normal subcutaneous fat cells from rat fat tissues. This system features an optimal condition of tissue dissociation system, Fat OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Fat PrimaCell™ 2 System (Cat No. 2-82544):
Rat Fat PrimaCell™ 2: Normal Subcutaneous Fat Cells Protocols
Rat Fat Tissue Preparation Buffer 2: Normal Subcutaneous Fat Cells
Rat Fat OptiTDS™ 2: Tissue Dissociation System
Rat Fat FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Rat Fat PrimaCell™ 2: Normal Subcutaneous Fat Cells Growth Medium
Rat Fat PrimaCell™ 2: Normal Subcutaneous Fat Cells Growth Supplements with Serum (for 500 ml medium)

References:

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[2] Jufvas A, Strålfors P, Vener AV. Histone variants and their post-translational modifications in primary human fat cells. PLoS One. 2011 Jan 7;6(1):e15960.
[3] Bujalska IJ, Durrani OM, Abbott J, Onyimba CU, Khosla P, Moosavi AH, Reuser TT, Stewart PM, Tomlinson JW, Walker EA, Rauz S. Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat. J Endocrinol. 2007 Feb;192(2):279-88.