The Rat Heart PrimaCell™ 2: Normal Atrial Myocytes is for rapid (7-10 days) isolation and growth of normal atrial myocytes from rat heart tissues. This system features an optimal condition of tissue dissociation system, Heart OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Heart PrimaCell™ 2 System (Cat No. 2-82547):
Rat Heart PrimaCell™ 2: Normal Atrial Myocytes Protocols
Rat Heart Tissue Preparation Buffer 2: Normal Atrial Myocytes
Rat Heart OptiTDS™ 2: Tissue Dissociation System
Rat Heart FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Rat Heart PrimaCell™ 2: Normal Atrial Myocytes Growth Medium
Rat Heart PrimaCell™ 2: Normal Atrial Myocytes Growth Supplements with Serum (for 500 ml medium)

References:

[1] Subedi KP, Singh TD, Kim JC, Woo SH. Cloning and expression of a new inositol 1,4,5-trisphosphate receptor type 1 splice variant in adult rat atrial myocytes. Cell Mol Biol Lett. 2012 Mar;17(1):124-35. Epub 2011 Dec 28.
[2] Qin Y, Zhang Z, Chen J, Ding X, Tong S, Song Z. Ca²⁺ disorder caused by rapid electrical field stimulation can be modulated by CaMKIIδ expression in primary rat atrial myocytes. Biochem Biophys Res Commun. 2011 Jun 3;409(2):287-92. Epub 2011 May 7.
[3] Bistola V, Nikolopoulou M, Derventzi A, Kataki A, Sfyras N, Nikou N, Toutouza M, Toutouzas P, Stefanadis C, Konstadoulakis MM. Long-term primary cultures of human adult atrial cardiac myocytes: cell viability, structural properties and BNP secretion in vitro. Int J Cardiol. 2008 Dec 17;131(1):113-22. Epub 2008 Feb 5.