The Rat Intestine PrimaCell™ 2: Normal Intestinal Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal intestinal smooth muscle cells from rat intestine tissues. This system features an optimal condition of tissue dissociation system, Intestine OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Intestine PrimaCell™ 2 System (Cat No. 2-82553):
Rat Intestine PrimaCell™ 2: Normal Intestinal Smooth Muscle Cells Protocols
Rat Intestine Tissue Preparation Buffer 2: Normal Intestinal Smooth Muscle Cells
Rat Intestine OptiTDS™ 2: Tissue Dissociation System
Rat Intestine FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Rat Intestine PrimaCell™ 2: Normal Intestinal Smooth Muscle Cells Growth Medium
Rat Intestine PrimaCell™ 2: Normal Intestinal Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Gauthier SA, Tizon B, Sahoo S, Levy E. In vitro assays measuring protection by proteins such as cystatin C of primary cortical neuronal and smooth muscle cells. Methods Mol Biol. 2012;849:275-87.
[2] Yang B, Gwozdz T, Dutko-Gwozdz J, Bolotina VM. Orai1 and Ca2+-independent phospholipase A2 are required for store-operated Icat-SOC current, Ca2+ entry, and proliferation of primary vascular smooth muscle cells. Am J Physiol Cell Physiol. 2012 Mar;302(5):C748-56. doi: 10.1152/ajpcell.00312.2011. Epub 2011 Nov 16.
[3] Halidi N, Boittin FX, Bény JL, Meister JJ. Propagation of fast and slow intercellular Ca(2+) waves in primary cultured arterial smooth muscle cells. Cell Calcium. 2011 Nov;50(5):459-67. doi: 10.1016/j.ceca.2011.08.001. Epub 2011 Sep 14.