The Rat Intestine PrimaCell™ 3: Normal Intestinal Vein Endothelial Cells is for rapid (7-10 days) isolation and growth of normal intestinal vein endothelial cells from rat intestine tissues. This system features an optimal condition of tissue dissociation system, Intestine OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Intestine PrimaCell™ 3 System (Cat No. 2-82554):
Rat Intestine PrimaCell™ 3: Normal Intestinal Vein Endothelial Cells Protocols
Rat Intestine Tissue Preparation Buffer 3: Normal Intestinal Vein Endothelial Cells
Rat Intestine OptiTDS™ 3: Tissue Dissociation System
Rat Intestine FibrOut™ 3: Fibroblast Inhibitory System (for 500 ml medium)
Rat Intestine PrimaCell™ 3: Normal Intestinal Vein Endothelial Cells Growth Medium
Rat Intestine PrimaCell™ 3: Normal Intestinal Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Tasnim F, Zink D. Cross talk between primary human renal tubular cells and endothelial cells in cocultures. Am J Physiol Renal Physiol. 2012 Apr;302(8):F1055-62. Epub 2012 Feb 8.
[2] Shkilnyy A, Proulx P, Sharp J, Lepage M, Vermette P. Diffusion of rhodamine B and bovine serum albumin in fibrin gels seeded with primary endothelial cells. Colloids Surf B Biointerfaces. 2012 May 1;93:202-7. Epub 2012 Jan 13.
[3] Mikkelsen L, Jensen KA, Koponen IK, Saber AT, Wallin H, Loft S, Vogel U, Møller P. Cytotoxicity, oxidative stress and expression of adhesion molecules in human umbilical vein endothelial cells exposed to dust from paints with or without nanoparticles. Nanotoxicology. 2012 Jan 20.