The Rat Kidney PrimaCell™ 1: Normal Glomerular Endothelial Cells is for rapid (7-10 days) isolation and growth of normal glomerular endothelial cells from rat kidney tissues. This system features an optimal condition of tissue dissociation system, Kidney OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Kidney PrimaCell™ 1 System (Cat No. 2-82555):
Rat Kidney PrimaCell™ 1: Normal Glomerular Endothelial Cells Protocols
Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells
Rat Kidney OptiTDS™ 1: Tissue Dissociation System
Rat Kidney FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Kidney PrimaCell™ 1: Normal Glomerular Endothelial Cells Growth Medium
Rat Kidney PrimaCell™ 1: Normal Glomerular Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Tasnim F, Zink D. Cross talk between primary human renal tubular cells and endothelial cells in cocultures. Am J Physiol Renal Physiol. 2012 Apr;302(8):F1055-62. Epub 2012 Feb 8.
[2] Pan Q, Yang XH, Cheng YX. Angiotensin II stimulates MCP-1 production in rat glomerular endothelial cells via NAD(P)H oxidase-dependent nuclear factor-kappa B signaling. Braz J Med Biol Res. 2009 Jun;42(6):531-6.