The Rat Kidney PrimaCell™ 2: Normal Kidney Epithelial Cells is for rapid (7-10 days) isolation and growth of normal kidney epithelial cells from rat kidney tissues. This system features an optimal condition of tissue dissociation system, Kidney OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Kidney PrimaCell™ 2 System (Cat No. 2-82556):
Rat Kidney PrimaCell™ 2: Normal Kidney Epithelial Cells Protocols
Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
Rat Kidney OptiTDS™ 2: Tissue Dissociation System
Rat Kidney FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Rat Kidney PrimaCell™ 2: Normal Kidney Epithelial Cells Growth Medium
Rat Kidney PrimaCell™ 2: Normal Kidney Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Sharpe CC, Dockrell ME. Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts. Methods Mol Biol. 2012;806:175-85.
[2] Valente MJ, Henrique R, Vilas-Boas V, Silva R, Bastos Mde L, Carvalho F, Guedes de Pinho P, Carvalho M. Cocaine-induced kidney toxicity: an in vitro study using primary cultured human proximal tubular epithelial cells. Arch Toxicol. 2012 Feb;86(2):249-61. Epub 2011 Oct 8.
[3] Domhan S, Ma L, Tai A, Anaya Z, Beheshti A, Zeier M, Hlatky L, Abdollahi A. Intercellular communication by exchange of cytoplasmic material via tunneling nano-tube like structures in primary human renal epithelial cells. PLoS One. 2011;6(6):e21283. Epub 2011 Jun 27.