The Rat Renal Tubular PrimaCell™ 7: Normal Renal Tubular Epithelial Cells is for rapid (7-10 days) isolation and growth of normal renal tubular epithelial cells from rat renal tubular tissues. This system features an optimal condition of tissue dissociation system, Renal Tubular OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Rat Renal Tubular PrimaCell™ 7 System (Cat No. 2-82562):
Rat Renal Tubular PrimaCell™ 7: Normal Renal Tubular Epithelial Cells Protocols
Rat Renal Tubular Tissue Preparation Buffer 7: Normal Renal Tubular Epithelial Cells
Rat Renal Tubular OptiTDS™ 7: Tissue Dissociation System
Rat Renal Tubular FibrOut™ 7: Fibroblast Inhibitory System (for 500 ml medium)
Rat Renal Tubular PrimaCell™ 7: Normal Renal Tubular Epithelial Cells Growth Medium
Rat Renal Tubular PrimaCell™ 7: Normal Renal Tubular Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Heutinck KM, Rowshani AT, Kassies J, Claessen N, van Donselaar-van der Pant KA, Bemelman FJ, Eldering E, van Lier RA, Florquin S, Ten Berge IJ, Hamann J. Viral double-stranded RNA sensors induce antiviral, pro-inflammatory, and pro-apoptotic responses in human renal tubular epithelial cells. Kidney Int. 2012 May 30. doi: 10.1038/ki.2012.206.
[2] Tasnim F, Zink D. Cross talk between primary human renal tubular cells and endothelial cells in cocultures. Am J Physiol Renal Physiol. 2012 Apr;302(8):F1055-62. Epub 2012 Feb 8.
[3] Domhan S, Ma L, Tai A, Anaya Z, Beheshti A, Zeier M, Hlatky L, Abdollahi A. Intercellular communication by exchange of cytoplasmic material via tunneling nano-tube like structures in primary human renal epithelial cells. PLoS One. 2011;6(6):e21283. Epub 2011 Jun 27.