The Rat Lymph PrimaCell™ 1: Normal Endothelial Cells is for rapid (7-10 days) isolation and growth of normal endothelial cells from rat lymph tissues. This system features an optimal condition of tissue dissociation system, Lymph OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Rat Lymph PrimaCell™ 1 System (Cat No. 2-82570):
Rat Lymph PrimaCell™ 1: Normal Endothelial Cells Protocols
Rat Lymph Tissue Preparation Buffer 1: Normal Endothelial Cells
Rat Lymph OptiTDS™ 1: Tissue Dissociation System
Rat Lymph FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Lymph PrimaCell™ 1: Normal Endothelial Cells Growth Medium
Rat Lymph PrimaCell™ 1: Normal Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
References:
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[2] Coso S, Zeng Y, Sooraj D, Williams ED. Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells. Exp Cell Res. 2011 Oct 15;317(17):2397-407. Epub 2011 Aug 2.
[3] Ji RC. Lymphatic endothelial cells, lymphedematous lymphangiogenesis, and molecular control of edema formation. Lymphat Res Biol. 2008;6(3-4):123-37. Review.