The Rat Muscle PrimaCell™ 2: Normal Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal smooth muscle cells from rat muscle tissues. This system features an optimal condition of tissue dissociation system, Muscle OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Muscle PrimaCell™ 2 System (Cat No. 2-82574):
Rat Muscle PrimaCell™ 2: Normal Smooth Muscle Cells Protocols
Rat Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells
Rat Muscle OptiTDS™ 2: Tissue Dissociation System
Rat Muscle FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Rat Muscle PrimaCell™ 2: Normal Smooth Muscle Cells Growth Medium
Rat Muscle PrimaCell™ 2: Normal Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Olesen M, Skov V, Mechta M, Mumm BH, Rasmussen LM. No influences of OPG and its ligands, RANKL and TRAIL, on proliferation and regulation of the calcification process in primary human vascular smooth muscle cells. Mol Cell Endocrinol. 2012 Jun 12.
[2] Van Ly D, Burgess JK, Brock TG, Lee TH, Black JL, Oliver BG. Prostaglandins but not leukotrienes alter extracellular matrix protein deposition & cytokine release in primary human airway smooth muscle cells & fibroblasts. Am J Physiol Lung Cell Mol Physiol. 2012 May 25.
[3] Gauthier SA, Tizon B, Sahoo S, Levy E. In vitro assays measuring protection by proteins such as cystatin C of primary cortical neuronal and smooth muscle cells. Methods Mol Biol. 2012;849:275-87.