The Rat Skin PrimaCell™ 2: Normal Melanocytes is for rapid (7-10 days) isolation and growth of normal melanocytes from rat skin tissues. This system features an optimal condition of tissue dissociation system, Skin OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Skin PrimaCell™ 2 System (Cat No. 2-82583):
Rat Skin PrimaCell™ 2: Normal Melanocytes Protocols
Rat Skin Tissue Preparation Buffer 2: Normal Melanocytes
Rat Skin OptiTDS™ 2: Tissue Dissociation System
Rat Skin FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Rat Skin PrimaCell™ 2: Normal Melanocytes Growth Medium
Rat Skin PrimaCell™ 2: Normal Melanocytes Growth Supplements with Serum (for 500 ml medium)

References:

[1] Qiao S, Lamore SD, Cabello CM, Lesson JL, Muñoz-Rodriguez JL, Wondrak GT. Thiostrepton is an inducer of oxidative and proteotoxic stress that impairs viability of human melanoma cells but not primary melanocytes. Biochem Pharmacol. 2012 May 1;83(9):1229-40. Epub 2012 Feb 1.
[2] Tam I, Stępień K. Secretion of proinflammatory cytokines by normal human melanocytes in response to lipopolysaccharide. Acta Biochim Pol. 2011;58(4):507-11. Epub 2011 Nov 30.
[3] Li L, Fukunaga-Kalabis M, Herlyn M. Isolation and cultivation of dermal stem cells that differentiate into functional epidermal melanocytes. Methods Mol Biol. 2012;806:15-29.