Gene Subcloning
CHI Scientific offers broad subcloning service that is highly flexible to any specific project requirements. The starting materials can be plasmid DNA, bacterial culture or simply just a gene name. Please contact our customer service for details. Our typical turnaround is 14 business days depending on the nature of the subcloning project.
Sample Submission Requirements
For project involved with plasmid DNA, and bacterial culture, please submit your samples according to the following guidance.
The complete sequence of the template including the target gene and its vector
Maps and antibiotic resistance of the template and destination vectors
Recommended to send 2 μg of template DNA as the starting material. If the starting material is bacterial culture, please send the culture at sufficient amount.
Delivery Specifications
Our typical turnaround is about 14 business days for < 3 kb genes depending on the complexity of the subcloning project. Our delivery package includes the following:
10 μg lyophilized destination vector containing the gene
Sequence chromatograms covering your gene (electronic)
Construct map for the plasmid (electronic)
Certificate of Analysis